human fasl cd95l duoset elisa  (R&D Systems)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Identification and Verification of Necroptosis‐Related Genes in Patients With Sepsis by Bioinformatic Analysis and Molecular Experiments

doi: 10.1111/jcmm.70582

Figure Lengend Snippet: Study flow diagram. This figure was created with BioRender.com. ELISA, enzyme‐linked immunosorbent assay; qPCR, quantitative real‐time polymerase chain reaction; SMC‐RoCI, Samsung Medical Center Registry of Critical Illness.

Article Snippet: The FasL levels were measured using the Human FasL ELISA Kit (R&D Systems, Minneapolis, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

Journal: Journal of Cellular and Molecular Medicine

Article Title: Identification and Verification of Necroptosis‐Related Genes in Patients With Sepsis by Bioinformatic Analysis and Molecular Experiments

doi: 10.1111/jcmm.70582

Figure Lengend Snippet: Comparison of eight differentially expressed genes, grouped into both immune system‐related and necroptosis‐related categories, plus corresponding proteins between the sepsis and control groups.

Article Snippet: The FasL levels were measured using the Human FasL ELISA Kit (R&D Systems, Minneapolis, USA).

Techniques: Comparison, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Identification and Verification of Necroptosis‐Related Genes in Patients With Sepsis by Bioinformatic Analysis and Molecular Experiments

doi: 10.1111/jcmm.70582

Figure Lengend Snippet: Comparison of the levels of proteins related to necroptosis‐related differentially expressed genes between the sepsis and control groups and between the deceased and surviving patients in the sepsis group (ELISA analysis).

Article Snippet: The FasL levels were measured using the Human FasL ELISA Kit (R&D Systems, Minneapolis, USA).

Techniques: Comparison, Control, Enzyme-linked Immunosorbent Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Identification and Verification of Necroptosis‐Related Genes in Patients With Sepsis by Bioinformatic Analysis and Molecular Experiments

doi: 10.1111/jcmm.70582

Figure Lengend Snippet: Graphs showing receiver operating characteristic curves of proteins corresponding to the upregulated and downregulated necroptosis‐related genes for diagnosing sepsis in all study participants ( n = 145) (A) and predicting mortality in patients with sepsis ( n = 133) (B). Protein levels were measured using an enzyme‐linked immunosorbent assay. AUC, area under the curve; CYLD, Cylindromatosis Lysine 63 deubiquitinase; FADD, Fas associated via death domain; FasL, Fas ligand; NLRP6, NLR family pyrin domain containing 6; PYGL, glycogen phosphorylase L; TLR3, toll‐like receptor 3; TNF, tumour necrosis factor.

Article Snippet: The FasL levels were measured using the Human FasL ELISA Kit (R&D Systems, Minneapolis, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Identification and Verification of Necroptosis‐Related Genes in Patients With Sepsis by Bioinformatic Analysis and Molecular Experiments

doi: 10.1111/jcmm.70582

Figure Lengend Snippet: Quantification of corresponding proteins to the eight necroptosis‐related differentially expressed genes between patients with sepsis and healthy volunteers. The protein expression levels were measured in three cells, comprising HUVEC, Jurkat T and THP‐1 cells, with media transfected with plasma from sepsis patients and healthy volunteers for 24 h. Statistical significance was determined by comparing the protein expression level in each cell medium between patients with sepsis and healthy volunteers (* p < 0.05 and ** p < 0.01). CYLD, Cylindromatosis Lysine 63 deubiquitinase; FADD, Fas associated via death domain; FasL, Fas ligand; NLRP6, NLR family pyrin domain containing 6; PYGL, glycogen phosphorylase L; TLR3, toll‐like receptor 3; TNF, tumour necrosis factor.

Article Snippet: The FasL levels were measured using the Human FasL ELISA Kit (R&D Systems, Minneapolis, USA).

Techniques: Expressing, Transfection, Clinical Proteomics

Journal: Journal of Cellular and Molecular Medicine

Article Title: Identification and Verification of Necroptosis‐Related Genes in Patients With Sepsis by Bioinformatic Analysis and Molecular Experiments

doi: 10.1111/jcmm.70582

Figure Lengend Snippet: Schematic representation of the necroptosis pathway. Five upregulated necroptosis‐related differentially expressed genes that were identified and verified in this study are presented in grey colour. TNFR1 activation by TNF results in the complex formation between RIPK1 and TRADD. Under conditions of caspase‐8 inhibition, necroptosis occurs through the formation of the necrosome, which is a three‐protein complex (RIPK1, RIPK3 and FADD). The necrosome activates MLKL and phospho‐MLKL oligomerizes and translocates into the plasma membrane, where it causes membrane rupture and release of damage‐associated molecular patterns. Alternatively, the activation of Fas or receptor/ligand interaction, such as TLR3/LPS, also results in necroptosis. Casp8, caspase‐8; cFLIP, cellular FLICE (FADD‐like IL‐1β‐converting enzyme)‐inhibitory protein; FADD, Fas‐associated death domain; FasL, Fas ligand; LPS, lipopolysaccharide; MLKL, mixed‐lineage kinase domain‐like; PYGL, glycogen phosphorylase L; RIPK1, receptor‐interacting protein kinase 1; RIPK3, receptor‐interacting protein kinase 3; TLR3, toll‐like receptor 3; TLR4, toll‐like receptor 4; TNF, tumour necrosis factor; TNFR1, TNF receptor 1; TRADD, TNFR1‐associated death domain; TRAM, TRIF‐related adaptor molecule; TRIF, TIR domain‐containing adaptor protein.

Article Snippet: The FasL levels were measured using the Human FasL ELISA Kit (R&D Systems, Minneapolis, USA).

Techniques: Activation Assay, Inhibition, Clinical Proteomics, Membrane